His | 水果好營養
2013年9月7日—c-termHistagforpurificationandidentificationofproteinofinterest.DesignNotes.TheHis-Tag(or6XHis)isawidelyusesequencebothas ...
His-Tag
c-term His tag for purification and identification of protein of interest.
Design NotesThe His-Tag (or 6XHis) is a widely use sequence both as a reporter (using anti-His tag antibodies such as His-probe) and as purification tag.
the 6 histidine sequence has a very high affinity to nickel(II) ions and therefore is used in affinity purification columns with immobilized nickel ions. the binding of the His-Tag to the nickel ions is not efficient at acidic pH values so any purification process should be done above pH=7 (usually done at pH=8). Imidazole is used In order to elute the protein from column by competing with the His tag on binding to the immobilized Nickel ions.
His-Tag binding resins are commercially available and are widely used in recombinant protein purification processes.
this part contains the 6XHis sequence betw...
His-Tag | 水果好營養
Polyhistidine | 水果好營養
pAB | 水果好營養
What should be the DNA sequence of 6xHis tag? | 水果好營養
His | 水果好營養
A Look into the 6XHis Tag and its Uses | 水果好營養
His | 水果好營養
His | 水果好營養
Tagging the Expressed Protein with 6 Histidines | 水果好營養
Polyhistidine | 水果好營養
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