Ninhydrin test: Principle, Reaction | 水果好營養
![Ninhydrin test: Principle, Reaction](https://i.imgur.com/Dp6k9gg.jpg)
2020年4月3日—Ninhydrinismostcommonlyusedasaforensicchemicaltodetect“fingerprints”,asaminesleftoverfromproteinssloughedoffinfingerprints ...
![Ninhydrin test: Principle, Reaction](https://i.imgur.com/Dp6k9gg.jpg)
Ninhydrin test
Detection of all amino acids, peptides and proteins
Ninhydrin (triketohydrindene hydrate) is used to assess amino acid qualitatively and quantitatively in ninhydrin reaction. All amino acids having α amino group, when heated in excess of ninhydrin give a purple coloured product. Only proline and hydroxyproline- amino acids molecules which contain a secondary amino group produce yellow condensation product. The colour intensity is proportional to the concentration of amino acid i.e. ammonia from the amino groups.
Image source: https://byjus.com/chemistry/ninhydrin-test/
Proteins also contain free amino groups on the alpha carbon and can react with ninhydrin to produce a blue-purple product. That is why the reaction with ninhydrin is u...
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