GSHGSSG Ratio Detection Assay Kit (Fluorometric | 水果好營養
![GSHGSSG Ratio Detection Assay Kit (Fluorometric](https://i.imgur.com/Dp6k9gg.jpg)
Inhealthycells,>90%ofthetotalglutathionepoolisinthereducedform(GSH).Whencellsareexposedtoincreasedlevelsofoxidativestress,GSSG ...
![GSHGSSG Ratio Detection Assay Kit (Fluorometric](https://i.imgur.com/Dp6k9gg.jpg)
For lysis, you can use 0.5% NP40 made up in PBS pH6.0 to lyse your cells. Please do not use the assay buffer to lyse your cells as it contains no detergent.
The deproteinization step is optional, however, we do find that it reduces interference and background in most samples.
With regards to the number of cells to use, this will depend largely on your cells and how much GSH/GSSG is present in your samples. Samples should ideally fall within the middle of standard curve so you may need to try a series of dilutions to determine the optimal conditions to use. As a starting point, the range of cell concentrations that we would recommend would be 10,000 to 100,000 cells/ well/100 uL. Try starting with ˜10,000 to 20,000 cells with adherent cells, and 50,000 to 100,000 cells with suspension cells.
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GSSGGSH ratios in cryopreserved rat and human ... | 水果好營養
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GSHGSSG Ratio Detection Assay Kit (Fluorometric | 水果好營養
GSSGGSH ratios in cryopreserved rat and human ... | 水果好營養
![](https://i.imgur.com/Dp6k9gg.jpg)
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