Bradford Assay (Bradford Reagent) | 水果好營養
![Bradford Assay (Bradford Reagent)](https://i.imgur.com/Dp6k9gg.jpg)
Bradfordassaysarecoomassiedye-bindingassaysforfastandsimpleproteinquantification.Theassayisperformedatroomtemperatureandnospecial ...
![Bradford Assay (Bradford Reagent)](https://i.imgur.com/Dp6k9gg.jpg)
The Bradford assay was first described by Dr. Marion Bradford in 1976 and uses Coomassie G-250 dye in a colorimetric reagent for the detection and quantitation of total protein. Pierce Bradford Plus Protein Assays are modifications of the reagent first reported by Dr. Bradford.
Chemistry of Coomassie-based Bradford protein assaysIn an acidic environment, proteins bind to Coomassie dye. This results in a spectral shift from the reddish-brown form of the dye (absorbance maximum at 465 nm) to the blue form (absorbance maximum at 610 nm). The difference between the two dye forms is greatest at 595 nm, making it the optimal wavelength to measure the blue color from the Coomassie dye–protein complex. If desired, the blue color can be measured at any wavelength between 575 nm and 615 nm. At the two extremes (575 nm and 615 nm), there is an approximate 10% decrease in the measured amount of color (absorb...
Bradford Assay (Bradford Reagent) | 水果好營養
Bradford Protein Assay | 水果好營養
Bradford Protein Assay | 水果好營養
Bradford Protein Assay | 水果好營養
Bradford protein assay | 水果好營養
Dual-RangeTM Bradford Protein Assay Kit | 水果好營養
布拉德福蛋白質定量法 | 水果好營養
產品介紹- | 水果好營養
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