His-Tag Purification | 水果好營養
His-tagpurificationusesthepurificationtechniqueofimmobilizedmetalaffinitychromatography,orIMAC.Inthistechnique,transitionmetalionsare ...
How Does His-Tag Purification Work?A his-tag, or polyhistidine tag, is a string of histidine residues at either the N or C terminus of a recombinant protein. There can be from four to ten residues in a string, although commonly there are six histidine residues — a hexahistidine tag. Some recombinant proteins are engineered to have two hexahistidine tags.
His-tag purification uses the purification technique of immobilized metal affinity chromatography, or IMAC. In this technique, transition metal ions are immobilized on a resin matrix using a chelating agent such as iminodiacetic acid. The most common ion for his-tag purification of a recombinant protein is Ni2+, though Co2+, Cu2+, and Zn2+ are also used. The his-tag has a high affinity for these metal ions and binds strongly to the IMAC column. Most other proteins in the lysate will not bind to the resin, or bind only weakly. The use of a his...
cOmplete™ His | 水果好營養
Expression and purification of proteins using 6xHistidine | 水果好營養
His Tagged Protein Purification | 水果好營養
His | 水果好營養
His-Tag Purification | 水果好營養
His | 水果好營養
Histagged protein purification protocol | 水果好營養
Purification of 6x His-tagged Protein (from E. coli) | 水果好營養
Purification of His | 水果好營養
蟠桃組胺酸(His)含量多少?
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