His | 水果好營養
AffinitypurificationofHis-taggedfusionproteinsisthemostcommonapplicationformetal-chelatesupportsinproteinbiologyresearch.Nickelorcobalt ...
The histidine tagThe DNA sequence specifying a string of six to nine histidine residues is frequently used in vectors for production of recombinant proteins. The result is expression of a recombinant protein with a 6xHis or poly-His-tag fused to its N- or C-terminus.
Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. In addition, anti-His-tag antibodies are commercially available for use in assay methods involving His-tagged proteins. In either case, the tag provides a means of specifically purifying or detecting the recombinant protein without a protein-specific antibody or probe.
Recombinant Protein Purification Selection ...
cOmplete™ His | 水果好營養
Expression and purification of proteins using 6xHistidine | 水果好營養
His Tagged Protein Purification | 水果好營養
His | 水果好營養
His-Tag Purification | 水果好營養
His | 水果好營養
Histagged protein purification protocol | 水果好營養
Purification of 6x His-tagged Protein (from E. coli) | 水果好營養
Purification of His | 水果好營養
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