His | 水果好營養
PurificationofHis-Tagproteinsisbysinglestepimmobilizedmetalaffinitychromatography(IMAC);aspecializedformofaffinitychromatographywhere ...
A His-tag enables easy purification of your protein of interest
Introduction The study of protein regulation, structure, and function relies heavily on the expression[1] and purification of recombinant proteins. Many recombinant proteins are expressed as fusion proteins, meaning that they contain an affinity/epitope tag (e.g. His[2] or GST). A tag is a short sequence of DNA that codes for a specific amino acid, which is frequently inserted into a target gene at the point of coding for expression at either the N or C terminal of the protein required.
One of the most commonly used tags is the polyhistidine tag, also known as His-Tag[3], which is a string of usually between six and nine histidine residues (see Figure 1 below). This method of tagging is especially useful as it allows for easy purification and detectio...
cOmplete™ His | 水果好營養
Expression and purification of proteins using 6xHistidine | 水果好營養
His Tagged Protein Purification | 水果好營養
His | 水果好營養
His-Tag Purification | 水果好營養
His | 水果好營養
Histagged protein purification protocol | 水果好營養
Purification of 6x His-tagged Protein (from E. coli) | 水果好營養
Purification of His | 水果好營養
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